The Development and Evaluation of Reagentless Glucose Biosensors Using Dendritic Gold Nanostructures as a Promising Sensing Platform

Reagentless electrochemical glucose biosensors were developed and investigated. A graphite rod (GR) electrode modified with electrochemically synthesized dendritic gold nanostructures (DGNs) redox mediators (Med) such as ferrocenecarboxylic acid (FCA), 1,10-phenathroline-5,6-dione (PD), N,N,N′,N′-tetramethylbenzidine (TMB) or tetrathiafulvalene (TTF) in combination oxidase (GOx) (GR/DGNs/FCA/GOx, GR/DGNs/PD/GOx, GR/DGNs/TMB/GOx, GR/DGNs/TTF/GOx) biosensor based on threefold-layer-by-layer-deposited PD GOx (GR/DGNs/(PD/GOx)3) was found to be the most suitable for determination of glucose. To improve performance biosensor, surface GR/DGNs/(PD/GOx)3 polypyrrole (Ppy) 5 h. a GR/DGNs/(PD/GOx)3/Ppy(5 h) characterized using wide linear dynamic range up 39.0 mmol L−1 glucose, sensitivity 3.03 µA mM−1 cm−2, limit detection 0.683 L−1, repeatability 9.03% 29.4 concentration. The Ppy-based by good storage stability (τ1/2 = 9.0 days). Additionally, blood serum